thermo multiskan ascent software Thermo Multiskan Ascent Software 17 Download ... an ELISA reader (Multiskan Ascent, Labsystem, Vantaa, Finland) at 450 nm. 2.8. Th1-Th17 Phenotipagem Spleens were aseptically removed and splenocytes were ... permeabilized using eBiosciences' intracellular fixation (Thermo Scientific, ... (BD Biosciences) and analyzed with the flow cytometer's proprietary software..